Electron microscopy in microbiology
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Electron microscopy in microbiology

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Published by Academic Press in London, Orlando, Fla .
Written in English

Subjects:

  • Electron microscopy,
  • Molecular biology

Book details:

Edition Notes

Includes bibliographies and index.

Statementedited by F. Mayer.
SeriesMethods in microbiology -- v. 20
ContributionsMayer, Frank.
The Physical Object
Paginationx, 431 p. :
Number of Pages431
ID Numbers
Open LibraryOL14487551M
ISBN 100125215206
OCLC/WorldCa18998017

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Get this from a library! Electron microscopy in microbiology. [Michael Hoppert; A Holzenburg; Royal Microscopical Society (Great Britain)] -- Electron Microscopy in Microbiology is a practical guide for microbiologists with little or no previous experience of electron microscopical preparation techniques. Concise descriptions of protocols. This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists. It explains the mechanisms of image formation, contrast and its enhancement, and accounts for ultimate limits on the size of Cited by:   Electron microscopy uses a beam of electrons as an energy source. An electron beam has an exceptionally short wavelength and can hit most objects in its path, increasing the resolution of the final image captured. The electron beam is designed to travel in a . This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold.

In clinical virology, electron microscopy (EM) has achieved a role equivalent to that of conventional light microscopy in clinical microbiology. EM allows for the rapid detection of the virus in a clinical specimen, at least at the level of the family into which it is classified, with a very high degree of specificity. Immunoelectron microscopy (IEM) arose from the combination of EM with the Cited by: 6. Get this from a library! Electron microscopy in microbiology. [Frank Mayer;] -- This volume of this acclaimed series deals with electron microscopic techniques applied for the elucidation of microbial structures and structure-function relationships at cellular, sub-cellular, and. Whereas transmission electron microscopy requires very thin sections and allows one to see internal structures such as organelles and the interior of membranes, scanning electron microscopy can be used to view the surfaces of larger objects (such as a pollen grain) as well as the surfaces of very small samples (Figure ). Electron Microscopy covers all of the important aspects of electron microscopy for biologists, including theory of scanning and transmission, specimen preparation, digital imaging and image analysis, laboratory safety and interpretation of images. The text also contains a complete atlas of ultrastructure.4/5(4).

Electron microscope, microscope that attains extremely high resolution using an electron beam instead of a beam of light to illuminate the object of study. Fundamental research by many physicists in the first quarter of the 20th century suggested that cathode rays (i.e., electrons) might be used in. Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. This chapter explains some of the mysteries of electron microscopy (EM), and makes the various techniques more user friendly to researchers who have lost the skills and recognized the importance of its use. The first level of ultrastructural information is provided by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The energy source used in the electron microscope is a beam of electrons. The beam has an exceptionally short wavelength, and increases the resolution of the image significantly over light microscopy. Whole objects are coated in gold or palladium, which deflects the electron beam, creating dark and light areas as 3-D images viewed on a monitor.